Goat anti-SIT1 Antibody |
| Principle Name | SIT1 |
| Official Symbol | SIT1 |
| All Name and Symbols | SIT1; signaling threshold regulating transmembrane adaptor 1; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SIT; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adaptor protein; suppression inducing transmembrane adaptor 1 |
| Accession Number(s) | NP_055265.1 |
| Peptide Sequence | HLSQWTRGRSRSH, is from internal region |
| Human Gene ID(s) | 27240 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Preliminary experiments in Human Lymph Node, Spleen, Tonsil and Thymus lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results |
| Reference(s) | Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B, SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. The Journal of experimental medicine 1999 Apr 189 (8) |
