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Goat anti-Glutamate Dehydrogenase Antibody
Item#:
dAP-2035
Size Price Quantity
50.0 ug $310.00
Goat anti-Glutamate Dehydrogenase Antibody
Principle Name Glutamate Dehydrogenase
Official Symbol GLUD1 / GLUD2
All Name and Symbols GLUD1; glutamate dehydrogenase 1; GDH; GDH1; GLUD; MGC132003; glutamate dehydrogenase (NAD(P)+); GLUD2; glutamate dehydrogenase 2; GDH2; GLUDP1; glutamate dehydrogenase pseudogene 1
Accession Number(s) NP_005262.1; NP_036216.2
Peptide Sequence ESEEQKRNRVRGILR, is from internal region
Human Gene ID(s) 2746; 2747
Non-Human GeneID(s) 14661 (mouse); 24399 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IF, IHC
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa acco
Immunohistochemistry In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration, 3-5µg/ml.
Reference(s) Jozwik M, Pietrzycki B, Jozwik M, Anthony RV. Expression of enzymes regulating placental ammonia homeostasis in human fetal growth restricted pregnancies. Placenta. 2009 Jul;30(7)

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