Goat anti-BOD1 Antibody |
| Principle Name | BOD1 |
| Official Symbol | BOD1 |
| All Name and Symbols | BOD1; biorientation of chromosomes in cell division 1; FAM44B; biorientation defective 1; family with sequence similarity 44, member B |
| Accession Number(s) | NP_612378.1; NP_001153123.1 |
| Peptide Sequence | NFVSTHLDKQE, is from internal region |
| Human Gene ID(s) | 91272 |
| Non-Human GeneID(s) | 69556 (mouse); 287173 (rat); |
| Important Comments | This antibody is expected to recognize isoforms a and b (NP_612378.1; NP_001153123.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx 37kDa band in Human Lung and Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size |
| Immunohistochemistry | Paraffin embedded Human Placenta. Recommended concentration |
| Reference(s) | Porter IM, McClelland SE, Khoudoli GA, Hunter CJ, Andersen JS, McAinsh AD, Blow JJ, Swedlow JR, Bod1, a novel kinetochore protein required for chromosome biorientation. The Journal of cell biology 2007 Oct 179 (2) |
