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Goat anti-SPAG16 (aa139-152) Antibody
Item#:
dAP-2017
Size Price Quantity
50.0 ug $310.00
Goat anti-SPAG16 (aa139-152) Antibody
Principle Name SPAG16 (aa139-152)
Official Symbol SPAG16
All Name and Symbols SPAG16; sperm associated antigen 16; DKFZp666P1710; FLJ22724; FLJ37717; MGC87036; PF20; WDR29; WD repeat domain 29; sperm-associated WD repeat protein
Accession Number(s) NP_078808.3; NP_001020607.1
Peptide Sequence KGVTELRTVGNVPD, is from internal region
Human Gene ID(s) 79582
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kD
Immunohistochemistry This antibody was successfully used in Human and Murine frozen Brain sections (PMID
Reference(s) Zhang Z, Zariwala MA, Mahadevan MM, Caballero-Campo P, Shen X, Escudier E, Duriez B, Bridoux AM, Leigh M, Gerton GL, Kennedy M, Amselem S, Knowles MR, Strauss JF, A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme. Biology of reproduction 2007 Nov 77 (5)

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