Goat anti-VPREB3 Antibody

Item#:

dAP-2007

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-VPREB3 Antibody
Principle Name	VPREB3
Official Symbol	VPREB3
All Name and Symbols	VPREB3; pre-B lymphocyte 3; 8HS20; N27C7-2; pre-B lymphocyte gene 3
Accession Number(s)	NP_037510.1
Peptide Sequence	EAHNACVLTISPVQ, is from internal region
Human Gene ID(s)	29802
Non-Human GeneID(s)	22364 (mouse); 365550 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave an approx 26kDa band in Human Lymph Node lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13
Reference(s)	Rosnet O, Blanco-Betancourt C, Grivel K, Richter K, Schiff C, Binding of free immunoglobulin light chains to VpreB3 inhibits their maturation and secretion in chicken B cells. The Journal of biological chemistry 2004 Mar 279 (11)