Goat anti-DTX3 Antibody |
| Principle Name | DTX3 |
| Official Symbol | DTX3 |
| All Name and Symbols | DTX3; deltex 3 homolog (Drosophila); FLJ34766; MGC138863; MGC138864; RNF154; deltex 3 homolog |
| Accession Number(s) | NP_848597.1 |
| Peptide Sequence | KNKVTVSKPVWD, is from internal region (near N Terminus) |
| Human Gene ID(s) | 196403 |
| Non-Human GeneID(s) | 80904 (mouse); 500847 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Mouse, Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to |
| Immunohistochemistry | Paraffin embedded Human Brain (Cortex). Recommended concentration |
| Reference(s) | Takeyama K, Aguiar RC, Gu L, He C, Freeman GJ, Kutok JL, Aster JC, Shipp MA, The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity. The Journal of biological chemistry 2003 Jun 278 (24) |
