Goat anti-NTE Antibody |
| Principle Name | NTE |
| Official Symbol | PNPLA6 |
| All Name and Symbols | PNPLA6; patatin-like phospholipase domain containing 6; NTE; NTEMND; SPG39; sws; neuropathy target esterase |
| Accession Number(s) | NP_006693.3 |
| Peptide Sequence | YQGRQTSSIFEAAKQ, is from internal region |
| Human Gene ID(s) | 10908 |
| Non-Human GeneID(s) | 50767 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave an approx 75kDa band in lysates of cell lines HeLa and Kelly after 2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate |
| Reference(s) | Rainier S, Bui M, Mark E, Thomas D, Tokarz D, Ming L, Delaney C, Richardson
RJ, Albers JW, Matsunami N, Stevens J, Coon H, Leppert M, Fink JK. Neuropathy target esterase gene mutations cause motor neuron disease. Am J Hum Genet. 2008 Mar;82(3) |
