Goat anti-SMC5 Antibody |
| Principle Name | SMC5 |
| Official Symbol | SMC5 |
| All Name and Symbols | SMC5; structural maintenance of chromosomes 5; KIAA0594; SMC5L1; OTTHUMP00000063367; SMC5 protein; SMC5 structural maintenance of chromosomes 5-like 1 |
| Accession Number(s) | NP_055925.2 |
| Peptide Sequence | TSKHLEHKDNELRQ, is from Internal region |
| Human Gene ID(s) | 23137 |
| Non-Human GeneID(s) | 226026 (mouse); 293967 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments in Human Kidney and Liver lysates and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 2µg/ml). We would appreciate any feedback from people in the field - have |
| Reference(s) | Pebernard S, Perry JJ, Tainer JA, Boddy MN. Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability. Mol Biol Cell. 2008 Oct;19(10) |
