Goat anti-ACAT1 (aa253-266) Antibody

Item#:

dAP-1969

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ACAT1 (aa253-266) Antibody
Principle Name	ACAT1 (aa253-266)
Official Symbol	ACAT1
All Name and Symbols	ACAT1; acetyl-Coenzyme A acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase
Accession Number(s)	NP_000010.1
Peptide Sequence	DEEYKRVDFSKVPK, is from internal region
Human Gene ID(s)	38
Non-Human GeneID(s)	110446 (mouse); 25014 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IF
Tested Species	Human, Mouse, Rat
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Approx 40kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1 and 44.81kDa to Mouse NP_659033.1). Recommended concentration
Reference(s)	Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2)