Goat anti-CXXC4 Antibody |
| Principle Name | CXXC4 |
| Official Symbol | CXXC4 |
| All Name and Symbols | CXXC4; CXXC finger 4; IDAX; MGC149872; Dvl-binding protein IDAX (inhibition of the Dvl and Axin complex); inhibition of the Dvl and Axin complex |
| Accession Number(s) | NP_079488.1 |
| Peptide Sequence | GTSLERTPVPSAEA, is from C-Terminus (near) |
| Human Gene ID(s) | 80319 |
| Non-Human GeneID(s) | 319478 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments in Human Brain (Cerebellum, Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been |
| Immunohistochemistry | In paraffin embedded Human Breast shows cystoplasm staining in myoepithelial cells. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Kojima T, Shimazui T, Hinotsu S, Joraku A, Oikawa T, Kawai K, Horie R, Suzuki H, Nagashima R, Yoshikawa K, Michiue T, Asashima M, Akaza H, Uchida K. Decreased expression of CXXC4 promotes a malignant phenotype in renal cell carcinoma by activating Wnt signaling. Oncogene 2008 Oct. [Epub ahead of print].PMID |
