Goat anti-RNF7 Antibody |
| Principle Name | RNF7 |
| Official Symbol | RNF7 |
| All Name and Symbols | RNF7; ring finger protein 7; CKBBP1; ROC2; SAG; OTTHUMP00000180645; regulator of cullins 2; sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2; zinc RING finger protein SAG |
| Accession Number(s) | NP_055060.1 |
| Peptide Sequence | DACLRCQAENKQE, is from internal region |
| Human Gene ID(s) | 9616 |
| Non-Human GeneID(s) | 19823 (mouse); |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_055060.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration |
| Reference(s) | Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA, E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Molecular cell 2009 Feb 33 (4) |