Goat anti-CEP290 Antibody |
| Principle Name | CEP290 |
| Official Symbol | CEP290 |
| All Name and Symbols | CEP290; centrosomal protein 290kDa; 3H11Ag, BBS14, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16; CTCL tumor antigen se2-2; monoclonal antibody 3H11 antigen; nephrocytsin-6; prostate cancer antigen T21 |
| Accession Number(s) | NP_079390.3 |
| Peptide Sequence | RNSKHLKQQQYRAEN, is from internal region |
| Human Gene ID(s) | 80184 |
| Non-Human GeneID(s) | 216274 (mouse); |
| Important Comments | Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, ICC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcul |
| Reference(s) | Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A. Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice. J. Clin. Invest. 2008 May 118 (5) |
