Goat anti-ZO-1 Antibody |
| Principle Name | ZO-1 |
| Official Symbol | TJP1 |
| All Name and Symbols | TJP1; tight junction protein 1 (zona occludens 1); DKFZp686M05161; MGC133289; ZO-1; OTTHUMP00000174520; tight junction protein 1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein |
| Accession Number(s) | NP_003248.3; NP_783297.2 |
| Peptide Sequence | PKPTSQNQFSEHDKT, is from internal region |
| Human Gene ID(s) | 7082 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration |
| Reference(s) | Zhong Z, Deane R, Ali Z, Parisi M, Shapovalov Y, O'Banion MK, Stojanovic K, Sagare A, Boillee S, Cleveland DW, Zlokovic BV, ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat. Neurosci. 2008 Apr 11 (4) |
