Goat anti-MTHFR Antibody |
| Principle Name | MTHFR |
| Official Symbol | MTHFR |
| All Name and Symbols | MTHFR; 5,10-methylenetetrahydrofolate reductase (NADPH); OTTHUMP00000002367; OTTHUMP00000002368; methylenetetrahydrofolate reductase intermediate form |
| Accession Number(s) | NP_005948.3 |
| Peptide Sequence | NRPTQNARETEAP, is from C Terminus |
| Human Gene ID(s) | 4524 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx 28kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we |
| Immunohistochemistry | Paraffin embedded Human Spleen. Recommended concentration |
| Reference(s) | Bethke L, Webb E, Murray A, Schoemaker M, Feychting M, Lönn S, Ahlbom A, Malmer B, Henriksson R, Auvinen A, Kiuru A, Salminen T, Johansen C, Christensen HC, Muir K, McKinney P, Hepworth S, Dimitropoulou P, Lophatananon A, Swerdlow A, Houlston R. Functional polymorphisms in folate metabolism genes influence the risk of meningioma and glioma. Cancer Epidemiol. Biomarkers Prev. 2008 May 17 (5) |
