Goat anti-SATB1 Antibody |
| Principle Name | SATB1 |
| Official Symbol | SATB1 |
| All Name and Symbols | SATB1; SATB homeobox 1; DNA-binding protein SATB1; special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's) |
| Accession Number(s) | NP_002962.1 |
| Peptide Sequence | ESDEENRQKTRPRT, is from internal region |
| Human Gene ID(s) | 6304 |
| Non-Human GeneID(s) | 20230 (mouse); 316164 (rat); |
| Important Comments | Reported variants NP_001124482.1 and NP_002962.1 represent identical protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Experiments gave bands at approx. 110kDa and 85kDa in lysates of cell line MOLT4 after 0.3µg/ml antibody staining. The observed 110kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol |
| Immunohistochemistry | In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Tan JA, Sun Y, Song J, Chen Y, Krontiris TG, Durrin LK. SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage. The Journal of biological chemistry 2008 Jun 283 (26) |
