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Goat anti-UPF3A / RENT3A Antibody


Item#:
dAP-1933
Size Price Quantity
50.0 ug $310.00
Goat anti-UPF3A / RENT3A Antibody
Principle Name UPF3A / RENT3A
Official Symbol UPF3A
All Name and Symbols UPF3A; UPF3 regulator of nonsense transcripts homolog A (yeast); HUPF3A; RENT3A; UPF3; OTTHUMP00000018789; UPF3 regulator of nonsense transcripts homolog A
Accession Number(s) NP_075387.1; NP_542418.1
Peptide Sequence KETDKQKKIAEKE, is from internal region
Human Gene ID(s) 65110
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcula
Reference(s) Tarpey PS, Raymond FL, Nguyen LS, Rodriguez J, Hackett A, Vandeleur L, Smith R, Shoubridge C, Edkins S, Stevens C, O'Meara S, Tofts C, Barthorpe S, Buck G, Cole J, Halliday K, Hills K, Jones D, Mironenko T, Perry J, Varian J, West S, Widaa S, Teague J, Di Mutations in UPF3B, a member of the nonsense-mediated mRNA decay complex, cause syndromic and nonsyndromic mental retardation. Nature genetics 2007 Sep 39 (9)

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