Goat anti-EML5 Antibody |
| Principle Name | EML5 |
| Official Symbol | EML5 |
| All Name and Symbols | EML5; echinoderm microtubule associated protein like 5; DKFZp781D1122; EMAP-2; HuEMAP-2 |
| Accession Number(s) | NP_899243.1 |
| Peptide Sequence | QQKEPSIDERQG, is from internal region |
| Human Gene ID(s) | 161436 |
| Non-Human GeneID(s) | 319670 (mouse); 444982 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments in Human Brain (Cerebellum, Cerebral Cortex and Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). |
| Immunohistochemistry | Paraffin embedded Human Small Intestine. Recommended concentration |
| Reference(s) | O'Connor V, Houtman SH, De Zeeuw CI, Bliss TV, French PJ. Eml5, a novel WD40 domain protein expressed in rat brain. Gene 2004 Jul 336 (1) |
