Goat anti-GAL Antibody |
| Principle Name | GAL |
| Official Symbol | GAL |
| All Name and Symbols | GAL; galanin prepropeptide; GALN; GLNN; GMAP; MGC40167; galanin; galanin-message-associated peptide; galanin-related peptide |
| Accession Number(s) | NP_057057.2 |
| Peptide Sequence | HRSFSDKNGLTSK, is from internal region |
| Human Gene ID(s) | 51083 |
| Non-Human GeneID(s) | 14419 (mouse); 29141 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 17kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 13.3kDa according to Human NP_057057.2 and of 13.5kDa according to Mouse NP_034383.1 and Rat NP_150240.1). Recommended concentration |
| Immunohistochemistry | Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration |
| Reference(s) | Sugimoto T, Seki N, Shimizu S, Kikkawa N, Tsukada J, Shimada H, Sasaki K, Hanazawa T, Okamoto Y, Hata A, The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma. Genes, chromosomes & cancer 2009 Feb 48 (2) |
