Goat anti-MDFI Antibody |
| Principle Name | MDFI |
| Official Symbol | MDFI |
| All Name and Symbols | MDFI; MyoD family inhibitor; I-MF; I-mfa; OTTHUMP00000016375; OTTHUMP00000196414; inhibitor of MyoD family a |
| Accession Number(s) | NP_005577.1 |
| Peptide Sequence | SKSTTSQIPLQAQED, is from internal region |
| Human Gene ID(s) | 4188 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size |
| Immunohistochemistry | Paraffin embedded Human Lung (Bronchus). Recommended concentration |
| Reference(s) | Ma R, Rundle D, Jacks J, Koch M, Downs T, Tsiokas L, Inhibitor of myogenic family, a novel suppressor of store-operated currents through an interaction with TRPC1. The Journal of biological chemistry 2003 Dec 278 (52) |
