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Goat anti-PFKFB2 Antibody
Item#:
dAP-1911
Size Price Quantity
50.0 ug $310.00
Goat anti-PFKFB2 Antibody
Principle Name PFKFB2
Official Symbol PFKFB2
All Name and Symbols PFKFB2; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; DKFZp781D2217; MGC138308; MGC138310; PFK-2/FBPase-2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme; OTTHUMP00000034285; OTTHUMP00000034286; P
Accession Number(s) NP_006203.2; NP_001018063.1
Peptide Sequence SEQNNNSYETKTP, is from N Terminus
Human Gene ID(s) 5208
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa
Immunohistochemistry Paraffin embedded Human Spleen. Recommended concentration
Reference(s) Arden C, Hampson LJ, Huang GC, Shaw JA, Aldibbiat A, Holliman G, Manas D, Khan S, Lange AJ, Agius L, A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells. Biochem J. 2008 Apr 1;411(1)

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