Goat anti-NEFM / NF-M Antibody

Item#:

dAP-1909

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NEFM / NF-M Antibody
Principle Name	NEFM / NF-M
Official Symbol	NEFM
All Name and Symbols	NEFM; neurofilament, medium polypeptide; NEF3; NF-M; NFM; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium)
Accession Number(s)	NP_005373.2
Peptide Sequence	QASHITVERKDYLK, is from internal region
Human Gene ID(s)	4741
Non-Human GeneID(s)	18040 (mouse); 24588 (rat);
Important Comments	This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Mouse
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended co
Reference(s)	Levy E, Liem RK, D'Eustachio P, Cowan NJ Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein European J. Biochem. 1987 Jul 166 (1)