Goat anti-ICOS Antibody |
| Principle Name | ICOS |
| Official Symbol | ICOS |
| All Name and Symbols | ICOS; inducible T-cell co-stimulator; AILIM; CD278; MGC39850; activation-inducible lymphocyte immunomediatory molecule; inducible costimulator |
| Accession Number(s) | NP_036224.1 |
| Peptide Sequence | KGGQILCDLTKTKGS, is from internal region |
| Human Gene ID(s) | 29851 |
| Non-Human GeneID(s) | 54167 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the c |
| Immunohistochemistry | In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre. Recommended concentration |
| Reference(s) | Strauss L, Bergmann C, Szczepanski MJ, Lang S, Kirkwood JM, Whiteside TL, Expression of ICOS on human melanoma-infiltrating CD4+CD25highFoxp3+ T regulatory cells |
