Goat anti-Desmoglein 1 / DSG1 Antibody

Item#:

dAP-1900

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Desmoglein 1 / DSG1 Antibody
Principle Name	Desmoglein 1 / DSG1
Official Symbol	DSG1
All Name and Symbols	DSG1; desmoglein 1; CDHF4; DG1; DSG; desmosomal glycoprotein 1; pemphigus foliaceus antigen
Accession Number(s)	NP_001933.2
Peptide Sequence	YTTSDTLKPSVHVHD, is from internal region
Human Gene ID(s)	1828
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments in lysates of cell lines A431, HeLa, K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results b
Immunohistochemistry	In paraffin embedded Human Skin showsstaining of the cellular membranes of keratinocytes. Recommended concentration, 4-6µg/ml.
Reference(s)	Nilles L.A., Parry D.A., Powers E.E., Angst B.D., Wagner R.M., Green K.J Structural analysis and expression of human desmoglein