Goat anti-RAP80 Antibody |
| Principle Name | RAP80 |
| Official Symbol | UIMC1 |
| All Name and Symbols | UIMC1; ubiquitin interaction motif containing 1; RAP80; X2HRIP110; OTTHUMP00000161441; receptor associated protein 80; retinoid x receptor interacting protein |
| Accession Number(s) | NP_057374.3 |
| Peptide Sequence | TRRQKEAKTKSDSG, is from internal region |
| Human Gene ID(s) | 51720 |
| Non-Human GeneID(s) | 20184 (mouse); 290997 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 60kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literatu |
| Immunohistochemistry | Paraffin embedded Human Testis. Recommended concentration |
| Reference(s) | Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA. Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene 2009 Apr 28 (16) |
