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Goat anti-NEBL / nebulette Antibody
Item#:
dAP-1891
Size Price Quantity
50.0 ug $310.00
Goat anti-NEBL / nebulette Antibody
Principle Name NEBL / nebulette
Official Symbol NEBL
All Name and Symbols NEBL; nebulette; FLJ53769; LNEBL; MGC119746; MGC119747; bA56H7.1; LIM-nebulette; NEBL, actin-binding Z-disc protein; OTTHUMP00000019268; actin-binding Z-disk protein
Accession Number(s) NP_998734.1; NP_001166955.1
Peptide Sequence ELQRLKRTQE, is from internal region
Human Gene ID(s) 10529
Non-Human GeneID(s) 74103 (mouse);
Important Comments This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1µg/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. T
Reference(s) Bonzo JR, Norris AA, Esham M, Moncman CL, The nebulette repeat domain is necessary for proper maintenance of tropomyosin with the cardiac sarcomere. Exp Cell Res. 2008 Nov 15;314(19)

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