Goat anti-TPCN2 Antibody |
| Principle Name | TPCN2 |
| Official Symbol | TPCN2 |
| All Name and Symbols | TPCN2; two pore segment channel 2; FLJ41094; SHEP10; TPC2; two-pore calcium channel protein 2 |
| Accession Number(s) | NP_620714.2 |
| Peptide Sequence | EDELTERLSQHPH, is from C Terminus |
| Human Gene ID(s) | 219931 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx 30kDa band in Human Brain (Cerebral Cortex), Kidney and Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observ |
| Immunohistochemistry | Paraffin embedded Human Pancreas and Colon. Recommended concentration |
| Reference(s) | Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, Tang J, Rietdorf K, Teboul L, Chuang KT, Lin P, Xiao R, Wang C, Zhu Y, Lin Y, Wyatt CN, Parrington J, Ma J, Evans AM, Galione A, Zhu MX. NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature 2009 May 459 (7246) |