Goat anti-eIF5A Antibody |
| Principle Name | eIF5A |
| Official Symbol | EIF5A |
| All Name and Symbols | EIF5A; eukaryotic translation initiation factor 5A; EIF-5A; EIF5A1; MGC104255; MGC99547; eIF5AI; OTTHUMP00000128384; uORF A |
| Accession Number(s) | NP_001137232.1 |
| Peptide Sequence | RPPHRASFLKRLESK, is from internal region (near N Terminus) |
| Human Gene ID(s) | 1984 |
| Non-Human GeneID(s) | |
| Important Comments | Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 20kDa band observed in MOLT4 lysates (calculated MW of 20.2kDa according to NP_001137232.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration |
| Reference(s) | Koettnitz K, Kappel B, Baumruker T, Hauber J, Bevec D The genomic structure encoding human initiation factor eIF-5A Gene. 1994 Jul 144 (2) |