Goat anti-HUWE1 / LASU1 Antibody

Item#:

dAP-1866

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HUWE1 / LASU1 Antibody
Principle Name	HUWE1 / LASU1
Official Symbol	HUWE1
All Name and Symbols	HUWE1; HECT, UBA and WWE domain containing 1; ARF-BP1; HECTH9; HSPC272; Ib772; KIAA0312; LASU1; MULE; UREB1; ARF binding protein 1; BJ-HCC-24 tumor antigen; HECT domain protein LASU1; Mcl-1 ubiquitin ligase E3; OTTHUMP00000023356; OTTHUMP00000061860
Accession Number(s)	NP_113584.3
Peptide Sequence	DDLKSNTEYHKYQ, is from internal region
Human Gene ID(s)	10075
Non-Human GeneID(s)	59026 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 482kDa according to NP_113584.3. Therefore we cannot recommend an optimal concentration and the antibody i
Reference(s)	Stankiewicz AR, Livingstone AM, Mohseni N, Mosser DD. Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Cell death and differentiation 2009 Apr 16 (4)