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Goat anti-UBR4 / ZUBR1 Antibody
Item#:
dAP-1863
Size Price Quantity
50.0 ug $310.00
Goat anti-UBR4 / ZUBR1 Antibody
Principle Name UBR4 / ZUBR1
Official Symbol UBR4
All Name and Symbols UBR4; ubiquitin protein ligase E3 component n-recognin 4; FLJ41863; KIAA0462; KIAA1307; RBAF600; ZUBR1; p600; retinoblastoma-associated factor 600; retinoblastoma-associated factor 600-like protein; zinc finger, UBR1 type 1
Accession Number(s) NP_065816.2
Peptide Sequence NTTRATSREEKNLQ, is from C Terminus
Human Gene ID(s) 23352
Non-Human GeneID(s) 69116 (mouse); 313658 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody i
Immunohistochemistry In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10µg/ml.
Reference(s) Lennerz V, Fatho M, Gentilini C, Frye RA, Lifke A, Ferel D, Wölfel C, Huber C, Wölfel T. The response of autologous T cells to a human melanoma is dominated by mutated neoantigens. Proceedings of the National Academy of Sciences of the United States of America 2005 Nov 102 (44)

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