Goat anti-SKA3 Antibody |
| Principle Name | SKA3 |
| Official Symbol | C13orf3 |
| All Name and Symbols | C13orf3; chromosome 13 open reading frame 3; MGC4832; RAMA1; SKA3 |
| Accession Number(s) | NP_659498.3 |
| Peptide Sequence | PLSKTNSSSNDLE, is from internal region |
| Human Gene ID(s) | 221150 |
| Non-Human GeneID(s) | 219114 (mouse); 361047 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave an approx 35kDa and 150kDa band in HeLa lysates after 2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46 |
| Immunohistochemistry | Paraffin embedded Human Placenta. Recommended concentration |
| Reference(s) | Theis M, Slabicki M, Junqueira M, Paszkowski-Rogacz M, Sontheimer J, Kittler R, Heninger AK, Glatter T, Kruusmaa K, Poser I, Hyman AA, Pisabarro MT, Gstaiger M, Aebersold R, Shevchenko A, Buchholz F. Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division. The EMBO journal 2009 May 28 (10) |
