Goat anti-SRF Antibody |
| Principle Name | SRF |
| Official Symbol | SRF |
| All Name and Symbols | SRF; serum response factor (c-fos serum response element-binding transcription factor); MCM1; OTTHUMP00000039820 |
| Accession Number(s) | NP_003122.1 |
| Peptide Sequence | QASPSRDSSTDLTQ, is from internal region |
| Human Gene ID(s) | 6722 |
| Non-Human GeneID(s) | 20807 (mouse); 501099 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in lit |
| Reference(s) | Raimundo N, Vanharanta S, Aaltonen LA, Hovatta I, Suomalainen A, Downregulation of SRF-FOS-JUNB pathway in fumarate hydratase deficiency and in uterine leiomyomas. Oncogene 2009 Mar 28 (9) |
