Goat anti-PRMT5 Antibody |
| Principle Name | PRMT5 |
| Official Symbol | PRMT5 |
| All Name and Symbols | PRMT5; protein arginine methyltransferase 5; HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog |
| Accession Number(s) | NP_006100.2; NP_001034708.1 |
| Peptide Sequence | RACREKDRDPEAQ, is from internal region |
| Human Gene ID(s) | 10419 |
| Non-Human GeneID(s) | 27374 (mouse); 364382 (rat); |
| Important Comments | This antibody is expected to recognize both isoforms (NP_006100.2; NP_001034708.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 70kDa band observed in Jurkat lysates (calculated MW of 72.7kDa and 71.3kDa according to NP_006100.2; NP_001034708.1). Recommended concentration |
| Reference(s) | Zhao Q, Rank G, Tan YT, Li H, Moritz RL, Simpson RJ, Cerruti L, Curtis DJ, Patel DJ, Allis CD, Cunningham JM, Jane SM. PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing. Nature structural & molecular biology 2009 Mar 16 (3) |
