Goat anti-LIG1 / DNA ligase I Antibody

Item#:

dAP-1836

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-LIG1 / DNA ligase I Antibody
Principle Name	LIG1 / DNA ligase I
Official Symbol	LIG1
All Name and Symbols	LIG1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; DNA ligase I
Accession Number(s)	NP_000225.1
Peptide Sequence	RVREDKQPEQATTS, is from internal region (near C-Terminus)
Human Gene ID(s)	3978
Non-Human GeneID(s)	81513 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Reco
Reference(s)	Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A, Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites. J. Biol. Chem. 2003 Sep 278 (39)