Goat anti-SEPT6 Antibody |
| Principle Name | SEPT6 |
| Official Symbol | Sep-06 |
| All Name and Symbols | SEPT6; septin 6; KIAA0128; MGC16619; MGC20339; SEP2; SEPT2; septin 2 |
| Accession Number(s) | NP_665798.1; NP_055944.2; NP_665801.1 |
| Peptide Sequence | DEVNAFKQRKTA, is from internal region |
| Human Gene ID(s) | 23157 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | Approx 50kDa band observed in Human Testis, Thyroid Gland and MOLT4 cell lysates (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear
accumulation of NCK mediated by SOCS7. Cell. 2007 Sep 7;130(5) |