Goat anti-MEST Antibody |
| Principle Name | MEST |
| Official Symbol | MEST |
| All Name and Symbols | MEST; mesoderm specific transcript homolog (mouse); DKFZp686L18234; MGC111102; MGC8703; PEG1; mesoderm specific transcript; paternally expressed gene 1 |
| Accession Number(s) | NP_002393.2; NP_803490.1 |
| Peptide Sequence | QELLYRYKQNRSGR, is from internal region |
| Human Gene ID(s) | 4232 |
| Non-Human GeneID(s) | 17294 (mouse); 58827 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 37kDa band observed in Mouse and Rat Testis lysates (calculated MW of 37.6kDa according to Human NP_803490.1 and 38.8kDa according to Rat NP_001009617.1). Recommended concentration |
| Reference(s) | Li T, Vu TH, Lee KO, Yang Y, Nguyen CV, Bui HQ, Zeng ZL, Nguyen BT, Hu JF, Murphy SK, Jirtle RL, Hoffman AR, An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. J. Biol. Chem. 2002 Apr 277 (16) |
