Goat anti-MECL1 Antibody |
| Principle Name | MECL1 |
| Official Symbol | PSMB10 |
| All Name and Symbols | PSMB10; proteasome (prosome, macropain) subunit, beta type, 10; LMP10; MECL1; MGC1665; beta2i; macropain subunit MECl-1; multicatalytic endopeptidase complex subunit MECl-1; proteasome MECl-1; proteasome beta 10 subunit; proteasome catalytic subunit 2i; p |
| Accession Number(s) | NP_002792.1 |
| Peptide Sequence | PTEPVKRSGRYH, is from internal region |
| Human Gene ID(s) | 5699 |
| Non-Human GeneID(s) | 19171 (mouse); 291983 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 26kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9kDa according to NP_002792.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Tonsil shows efined staining in a subset of cells. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Moschonas A, Kouraki M, Knox PG, Thymiakou E, Kardassis D, Eliopoulos AG. CD40 induces antigen transporter and immunoproteasome gene expression in carcinomas via the coordinated action of NF-kappaB and of NF-kappaB-mediated de novo synthesis of IRF-1. Molecular and Cellular Biology 2008 Oct 28 (20) |
