Goat anti-PPP4C Antibody |
| Principle Name | PPP4C |
| Official Symbol | PPP4C |
| All Name and Symbols | PPP4C; protein phosphatase 4 (formerly X), catalytic subunit; PP4; PPH3; PPX; Protein phosphatase 4, catalytic subunit |
| Accession Number(s) | NP_002711.1 |
| Peptide Sequence | PQETRGIPSKKP, is from C Terminus |
| Human Gene ID(s) | 5531 |
| Non-Human GeneID(s) | 171366 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 35kDa band observed in HEK293, A431 and HeLa lysates and in Rat Spleen and Lung lysates (calculated MW of 35.1kDa according to NP_002711.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Mourtada-Maarabouni M, Williams GT. Protein phosphatase 4 regulates apoptosis, proliferation and mutation rate of human cells. Biochimica et biophysica acta 2008 Aug 1783 (8) |
