Goat anti-NCE2 / UBE2F Antibody

Item#:

dAP-1789

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NCE2 / UBE2F Antibody
Principle Name	NCE2 / UBE2F
Official Symbol	UBE2F
All Name and Symbols	UBE2F; ubiquitin-conjugating enzyme E2F (putative); MGC18120; NCE2; NEDD8 conjugating enzyme; NEDD8-conjugating enzyme
Accession Number(s)	NP_542409.1
Peptide Sequence	HLRDKEDFRNKVDD, is from C Terminus
Human Gene ID(s)	140739
Non-Human GeneID(s)	67921 (mouse); 363284 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx. 20kDa band observed in Mouse Liver lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration, 2.5-3.8µg/ml.
Reference(s)	Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol. Cell 33 (4), 483-495 (2009)..PMID