Goat anti-LIMP2 / SCARB2 Antibody |
| Principle Name | LIMP2 / SCARB2 |
| Official Symbol | SCARB2 |
| All Name and Symbols | SCARB2; scavenger receptor class B, member 2; AMRF; CD36L2; HLGP85; LIMPII; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane prote |
| Accession Number(s) | NP_005497.1 |
| Peptide Sequence | NKANIQFGDNGTTIS, is from internal region |
| Human Gene ID(s) | 950 |
| Non-Human GeneID(s) | 12492 (mouse); |
| Important Comments | This antibody is expected to recognize reported isoform 1 (NP_005497.1) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | Approx. 80kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et |
| Immunohistochemistry | In paraffin embedded Human Cerebral Cortex shows lysosomal staining. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Berkovic SF, Dibbens LM, Oshlack A, Silver JD, Katerelos M, Vears DF, Lüllmann-Rauch R, Blanz J, Zhang KW, Stankovich J, Kalnins RM, Dowling JP, Andermann E, Andermann F, Faldini E, D'Hooge R, Vadlamudi L, Macdonell RA, Hodgson BL, Bayly MA, Savige J, Mul Array-based gene discovery with three unrelated subjects shows SCARB2/LIMP-2 deficiency causes myoclonus epilepsy and glomerulosclerosis. Am. J. Hum. Genet. 2008 Mar 82 (3) |
