Goat anti-HIPPI Antibody |
| Principle Name | HIPPI |
| Official Symbol | IFT57 |
| All Name and Symbols | IFT57; intraflagellar transport 57 homolog (Chlamydomonas); ESRRBL1; FLJ10147; HIPPI; MHS4R2; HIP1 protein interactor; estrogen-related receptor beta like 1; huntingtin interacting protein-1 interacting protein |
| Accession Number(s) | NP_060480.1 |
| Peptide Sequence | EQPQEYDDPNATISN , is from internal region |
| Human Gene ID(s) | 55081 |
| Non-Human GeneID(s) | 73916 (mouse); 303968 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 49kDa band observed in Human and Mouse Brain lysates (calculated MW of 49.1kDa according to Human NP_060480.1 and of 48.8kDa according to Mouse NP_082956.2). Recommended concentration |
| Reference(s) | Niu Q, Ybe JA. Crystal structure at 2.8 A of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain reveals a charged surface suitable for HIP1 protein interactor (HIPPI). J. Mol. Biol. 2008 Feb 375 (5) |
