Goat anti-CHN2 Antibody |
| Principle Name | CHN2 |
| Official Symbol | CHN2 |
| All Name and Symbols | CHN2; chimerin (chimaerin) 2; ARHGAP3; RHOGAP3; RhoGAP3; beta chimerin; Chimerin 2 (GTPase-activating protein, rho, 3); BCH; MGC138360; rho-GTPase-activating protein 3 |
| Accession Number(s) | NP_001035025.1; NP_004058.1 |
| Peptide Sequence | QILIENEDVLF, is from C Terminus |
| Human Gene ID(s) | 1124 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_004058.1; NP_001035025.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Preliminary experiments in Human Brain (Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results |
| Immunohistochemistry | In paraffin embedded Human Cortex shows staining of neuronal bodies. Recommended concentration, 3-6µg/ml. |
| Reference(s) | Siliceo, M. and Merida, I. T cell receptor-dependent tyrosine phosphorylation of beta2-chimaerin modulates its Rac-GAP function in T cells. J. Biol. Chem. 284 (17), 11354-11363 (2009)..PMID |
