Goat anti-Klotho / KL Antibody

Item#:

dAP-1762

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Klotho / KL Antibody
Principle Name	Klotho / KL
Official Symbol	KL
All Name and Symbols	KL; klotho
Accession Number(s)	NP_004786.2
Peptide Sequence	NQSQVNHTILQYYRC, is from internal region
Human Gene ID(s)	9365
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 1000.
Western Blot	Preliminary experiments in Human Kidney, Pancreas, Prostate and Testis lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results
Reference(s)	Chen CD, Podvin S, Gillespie E, Leeman SE, Abraham CR. Insulin stimulates the cleavage and release of the extracellular domain of Klotho by ADAM10 and ADAM17. Proc Natl Acad Sci U S A. 2007 Dec 11;104(50)