Goat anti-TRC8 Antibody |
| Principle Name | TRC8 |
| Official Symbol | RNF139 |
| All Name and Symbols | RNF139; ring finger protein 139; TRC8; HRCA1; MGC31961; RCA1; patched related protein translocated in renal cancer; multiple membrane spanning receptor TRC8 |
| Accession Number(s) | NP_009149.2 |
| Peptide Sequence | HTGAAAEEFNDDT, is from C Terminus |
| Human Gene ID(s) | 11236 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 75kDa band observed in Human Skeletal Muscle lysates (calculated MW of 76.0kDa according to NP_009149.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Placenta shows membranous staining in the cytotrophoblasts. Recommended concentration, 3-4µg/ml. |
| Reference(s) | Gemmill RM, West JD, Boldog F, Tanaka N, Robinson LJ, Smith DI, Li F, Drabkin HA. The hereditary renal cell carcinoma 3;8 translocation fuses FHIT to a patched-related gene, TRC8. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16) |