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Goat anti-SLC10A2 / ASBT Antibody
Item#:
dAP-1755
Size Price Quantity
50.0 ug $310.00
Goat anti-SLC10A2 / ASBT Antibody
Principle Name SLC10A2 / ASBT
Official Symbol SLC10A2
All Name and Symbols SLC10A2; ASBT; solute carrier family 10 (sodium/bile acid cotransporter family), member 2; ISBT; NTCP2; OTTHUMP00000040691; ileal apical sodium-dependent bile acid transporter; ileal sodium-dependent bile acid transporter
Accession Number(s) NP_000443.1
Peptide Sequence YKANGGFQPDEK, is from C Terminus
Human Gene ID(s) 6555
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx. 60kDa band in Human Kidney lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.
Immunohistochemistry In paraffin embedded Human Small Intestine shows staining of cells in the laminia propria between the intestinal vili. Recommended concentration, 3-6µg/ml.
Reference(s) Annaba F, Sarwar Z, Kumar P, Saksena S, Turner JR, Dudeja PK, Gill RK, Alrefai WA. Modulation of ileal bile acid transporter (ASBT) activity by depletion of plasma membrane cholesterol

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