Goat anti-Synaptotagmin I Antibody

Item#:

dAP-1749

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Synaptotagmin I Antibody
Principle Name	Synaptotagmin I
Official Symbol	SYT1
All Name and Symbols	synaptotagmin I ; DKFZp781D2042; SYT1; P65; SVP65; SYT; synaptotagmin 1
Accession Number(s)	NP_005630.1
Peptide Sequence	RDLQSAEKEEQEK, is from internal region
Human Gene ID(s)	6857
Non-Human GeneID(s)	20979 (mouse); 25716 (rat);
Important Comments	Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibod
Immunohistochemistry	In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon. Recommended concentration
Reference(s)	Martens S, Kozlov MM, McMahon HT. How synaptotagmin promotes membrane fusion. Science. 2007 May 25;316(5828)