Goat anti-DGCR8 / Pasha Antibody

Item#:

dAP-1746

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-DGCR8 / Pasha Antibody
Principle Name	DGCR8 / Pasha
Official Symbol	DGCR8
All Name and Symbols	DGCR8; DiGeorge syndrome critical region gene 8; C22orf12; DGCRK6; Gy1; pasha
Accession Number(s)	NP_073557.3
Peptide Sequence	KRFDFEQVTVKKFRT, is from internal region
Human Gene ID(s)	54487
Non-Human GeneID(s)	94223 (mouse); 287954 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave an approx. 75kDa band in Human Brain (amygdala) lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated s
Reference(s)	Stark KL, Xu B, Bagchi A, Lai WS, Liu H, Hsu R, Wan X, Pavlidis P, Mills AA, Karayiorgou M, Gogos JA. Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat. Genet. 2008 Jun 40 (6)