Goat anti-OXT Antibody |
| Principle Name | OXT |
| Official Symbol | OXT |
| All Name and Symbols | OXT; oxytocin, prepro- (neurophysin I); MGC126890; MGC126892; OT; OT-NPI; oxytocin-neurophysin I; oxytocin-neurophysin I, preproprotein |
| Accession Number(s) | NP_000906.1 |
| Peptide Sequence | HADPACDAEATFSQR, is from C Terminus |
| Human Gene ID(s) | 5020 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | Preliminary experiments in Human Brain (Cerebellum, Amygdala) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been rep |
| Immunohistochemistry | Frozen section of Human Hypothalamus shows staining of magnocellular oxytocin neurons in the supraoptic nucleus. Recommended concentration, 0.05-0.15µg/ml. |
| Reference(s) | Meynen G, Unmehopa UA, Hofman MA, Swaab DF, Hoogendijk WJ. Hypothalamic oxytocin mRNA expression and melancholic depression. Mol Psychiatry. 2007 Feb;12(2) |
