Goat anti-ATG4D Antibody |
| Principle Name | ATG4D |
| Official Symbol | ATG4D |
| All Name and Symbols | ATG4D; ATG4 autophagy related 4 homolog D (S. cerevisiae); APG4-D; APG4D; AUTL4; APG4 autophagy 4 homolog D; AUT-like 4, cysteine endopeptidase; autophagin-4; autophagy-related cysteine endopeptidase 4; cysteine protease involved in autophagy |
| Accession Number(s) | NP_116274.3 |
| Peptide Sequence | CSQLAQPTLRLPRT, is from internal region (near C terminus) |
| Human Gene ID(s) | 84971 |
| Non-Human GeneID(s) | 235040 (mouse); 686505 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size |
| Immunohistochemistry | Paraffin embedded Human Pancreas. Recommended concentration |
| Reference(s) | Mariño G, Uría JA, Puente XS, Quesada V, Bordallo J, López-Otín C. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. The Journal of biological chemistry 2003 Feb 278 (6) |
