Goat anti-UNC5C Antibody |
| Principle Name | UNC5C |
| Official Symbol | UNC5C |
| All Name and Symbols | UNC5C; unc-5 homolog C (C. elegans); UNC5H3; homolog of C. elegans transmembrane receptor Unc5; unc5 (C.elegans homolog) c; unc5C |
| Accession Number(s) | NP_003719.2 |
| Peptide Sequence | NCTVSEEPTGID, is from internal region |
| Human Gene ID(s) | 8633 |
| Non-Human GeneID(s) | 22253 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IF, IHC |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with ot |
| Immunohistochemistry | In paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Shin SK, Nagasaka T, Jung BH, Matsubara N, Kim WH, Carethers JM, Boland CR, Goel A. Epigenetic and genetic alterations in Netrin-1 receptors UNC5C and DCC in human colon cancer. Gastroenterology 2007 Dec 133 (6) |
