Goat anti-THBS3 Antibody |
| Principle Name | THBS3 |
| Official Symbol | THBS3 |
| All Name and Symbols | THBS3; thrombospondin 3; MGC119564; MGC119565; TSP3; OTTHUMP00000033827 |
| Accession Number(s) | NP_009043.1 |
| Peptide Sequence | CNDTVPEDFEPFRRQ, is from C Terminus |
| Human Gene ID(s) | 7059 |
| Non-Human GeneID(s) | 21827 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the |
| Immunohistochemistry | In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2µg/ml. |
| Reference(s) | Pan MR, Chang HC, Chuang LY, Hung WC. The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells. Exp. Biol. Med. (Maywood) 2008 Apr 233 (4) |
